Polymerase Chain Reaction for Detection of Guinea Pig Adenovirus

Detection of Babesia ovis Using Polymerase Chain Reaction.

COMPARISON OF CULTURE WITH POLYMERASE CHAIN REACTION FOR DETECTION OF UREAPLASMA UREALYTICUM IN ENDOCERVICAL SPECIMENS

Background: Ureaplasma urealyticum is known as a sexually transmitted agent, causing mainly urethritis, pelvic int1ammatory disease, spontaneous abortion, pyelonephritis, infertility, stillbirth, low birth weight neonatal meningititis, and neonatal pneumonia. U. urealyticum infections not only jeopardize fertility but also pose a risk for infertility treatment and resulting pregnancies. Dia...

Polymerase Chain Reaction of Mgc2 and 16S rRNA Genes for Detection of Mycoplasma gallisepticum

Mycoplasmas are very small bacteria lacking cell walls that belong to various genera within the class Mollicutes, and also the smallest organisms that can live independently. They are able to cause serious and chronic disease because of some unique characteristics. Mycoplasma gallisepticum (MG) is an important avian pathogen causing significant economical losses within the poultry industry. The...

Polymerase chain reaction method for the rapid detection of virulent Shigella spp.

Bacillary dysentery, or shigellosis, is a disease of humans in which the colonic epithelium is invaded by bacteria and subjected to inflammatory destruction. The aim of this study was to develop a polymerase chain reaction(PCR) test for detection of virulent Shigella spp.. For this purpose, the primers were designed to amplify a 526-bp internal region of the Shigella spp. icsA gene, which encod...

Adenovirus polymerase chain reaction assay for rapid diagnosis of conjunctivitis.

PURPOSE To evaluate newly designed primers in a polymerase chain reaction (PCR) for the detection of adenovirus DNA in conjunctival swabs. METHODS Oligonucleotides were derived from the adenovirus hexon gene and modified such that a maximum of only two mismatches occurred with adenovirus types 2 through 5, 7, and 16. Specificity was determined against adenovirus types 2 through 4, 7, 8 throug...